There are two ways to optimize the antibody affinity of IKEST: single point complete random mutation combined with hot spot combination (PM) and segment complete random mutation (BM). The former is usually used for small affinity enhancement/minimal sequence modification; The latter is used for significant affinity maturation.
EKST can express antibody mutants in prokaryotic system and/or eukaryotic system. Both platforms have the ability of high-throughput expression and screening, significantly shortening the cycle of antibody affinity optimization.
EKST also has a unique hotspot scanning technology iPASS. This technology can quickly determine the position in the antibody sequence that has a gain effect on the affinity/antibody expression level, and further improve the efficiency of antibody modification.